Western blotting analysis using lysine demethylase 1 antibody (Cat#61567). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with lysine demethylase 1 antibody (Cat#61567, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using lysine demethylase 1 antibody (Cat#61567). Lysine demethylase 1 expression in wild-type (WT) and lysine demethylase 1 (KDM1A) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with lysine demethylase 1 antibody (Cat#61567, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using lysine demethylase 1A antibody (Cat#61567, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HepG2 cells with lysine demethylase 1 antibody (Cat#61567, 1:1,000). Nuclei were stained blue with DAPI; Lysine demethylase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Flow cytometric analysis of lysine demethylase 1 expression in HepG2 cells using Lysine demethylase 1 antibody (Cat#61567, 1:2,000). Green, isotype control; red, Lysine demethylase 1.
