Western blotting analysis using fibroblast growth factor receptor 1 antibody (Cat#61570). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with fibroblast growth factor receptor 1 antibody (Cat#61570, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using fibroblast growth factor receptor 1 antibody (Cat#61570). Fibroblast growth factor receptor 1 expression in wild type (WT) and fibroblast growth factor receptor 1 (FGFR1) knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with fibroblast growth factor receptor 1 antibody (Cat#61570, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using fibroblast growth factor receptor 1 antibody (Cat#61570, 1:200). Antigen retrieval was done in EDTA buffer (pH 9.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HAP-1 cells with fibroblast growth factor receptor 1 antibody (Cat#61570, 1:1,000). Nuclei were stained blue with DAPI; fibroblast growth factor receptor 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of fibroblast growth factor receptor 1 expression in HAP-1 cells using fibroblast growth factor receptor 1 antibody (Cat#61570, 1:2,000). Green, isotype control; red, Fibroblast growth factor receptor 1.
