Western blotting analysis using AMPK gamma 1 antibody (Cat#61582). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with AMPK gamma 1 antibody (Cat#61582, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using AMPK gamma 1 antibody (Cat#61582). AMPK gamma 1 expression in wild-type (WT) and PRKAG1 knockdown (KD) HSHC cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with AMPK gamma 1 antibody (Cat#61582, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using AMPK gamma 1 antibody (Cat#61582). AMPK gamma 1 expression in wild type (WT) and PRKAG1 knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with AMPK gamma 1 antibody (Cat#61582, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).