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  • Primary Antibody
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Home>Product>Primary Antibody

KD-Validated DDAH1 Recombinant Rabbit mAb

#61603

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Recombinant Antibody
Knockdown Validated
Western blotting analysis using dimethylarginine dimethylaminohydrolase 1 antibody (Cat#61603). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with dimethylarginine dimethylaminohydrolase 1 antibody (Cat#61603, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using dimethylarginine dimethylaminohydrolase 1 antibody (Cat#61603). Dimethylarginine dimethylaminohydrolase 1 expression in wild type (WT) and dimethylarginine dimethylaminohydrolase 1 (DDAH1) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with dimethylarginine dimethylaminohydrolase 1 antibody (Cat#61603, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Dimethylarginine dimethylaminohydrolase 1 expression in HT-1080 cells using Dimethylarginine dimethylaminohydrolase 1 antibody (Cat#61603, 1:2,000). Green, isotype control; red, Dimethylarginine dimethylaminohydrolase 1.
Western blotting analysis using dimethylarginine dimethylaminohydrolase 1 antibody (Cat#61603). Dimethylarginine dimethylaminohydrolase 1 expression in wild type (WT) and dimethylarginine dimethylaminohydrolase 1 (DDAH1) knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with dimethylarginine dimethylaminohydrolase 1 antibody (Cat#61603, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
20 μL 100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    DDAH1
  • Catalog：
    61603
  • Application：
    WB, FCM
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated DDAH1 Recombinant Rabbit mAb
Aliases DDAH1; Dimethylarginine Dimethylaminohydrolase 1; DDAH; N(G),N(G)-Dimethylarginine Dimethylaminohydrolase 1; Dimethylargininase-1; EC 3.5.3.18; DDAH-1; DDAHI; NG, NG-Dimethylarginine Dimethylaminohydrolase; Epididymis Secretory Protein Li 16; HEL-S-16
Background

Gene Name: DDAH1

NCBI Gene Entry: 23576

UniProt Entry: O94760

Application Information

Molecular Weight: Predicted, 31 kDa, observed, 36 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB2995

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen A synthesized peptide derived from human DDAH1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for 1 year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using dimethylarginine dimethylaminohydrolase 1 antibody (Cat#61603). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with dimethylarginine dimethylaminohydrolase 1 antibody (Cat#61603, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using dimethylarginine dimethylaminohydrolase 1 antibody (Cat#61603). Dimethylarginine dimethylaminohydrolase 1 expression in wild type (WT) and dimethylarginine dimethylaminohydrolase 1 (DDAH1) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with dimethylarginine dimethylaminohydrolase 1 antibody (Cat#61603, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Flow cytometric analysis of Dimethylarginine dimethylaminohydrolase 1 expression in HT-1080 cells using Dimethylarginine dimethylaminohydrolase 1 antibody (Cat#61603, 1:2,000). Green, isotype control; red, Dimethylarginine dimethylaminohydrolase 1.
  • Western blotting analysis using dimethylarginine dimethylaminohydrolase 1 antibody (Cat#61603). Dimethylarginine dimethylaminohydrolase 1 expression in wild type (WT) and dimethylarginine dimethylaminohydrolase 1 (DDAH1) knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with dimethylarginine dimethylaminohydrolase 1 antibody (Cat#61603, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Please select the specifications of the product.
Specification：
20 μL,100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    DDAH1
  • Catalog：
    61603
  • Application：
    WB, FCM
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using dimethylarginine dimethylaminohydrolase 1 antibody (Cat#61603). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with dimethylarginine dimethylaminohydrolase 1 antibody (Cat#61603, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using dimethylarginine dimethylaminohydrolase 1 antibody (Cat#61603). Dimethylarginine dimethylaminohydrolase 1 expression in wild type (WT) and dimethylarginine dimethylaminohydrolase 1 (DDAH1) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with dimethylarginine dimethylaminohydrolase 1 antibody (Cat#61603, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Dimethylarginine dimethylaminohydrolase 1 expression in HT-1080 cells using Dimethylarginine dimethylaminohydrolase 1 antibody (Cat#61603, 1:2,000). Green, isotype control; red, Dimethylarginine dimethylaminohydrolase 1.
Western blotting analysis using dimethylarginine dimethylaminohydrolase 1 antibody (Cat#61603). Dimethylarginine dimethylaminohydrolase 1 expression in wild type (WT) and dimethylarginine dimethylaminohydrolase 1 (DDAH1) knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with dimethylarginine dimethylaminohydrolase 1 antibody (Cat#61603, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Shopping cart (12)
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Estimated total order price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$23400
View the shopping cart Empty the shopping cart
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    support@genuinbiotech.com

    sales@genuinbiotech.com

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    8:30 AM - 5:30 PM EST Mon-Fri

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  133. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-orm/src/db/concern/TableFieldInfo.php ( 2.63 KB )
  134. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-orm/src/db/concern/Transaction.php ( 2.77 KB )
  135. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/log/driver/File.php ( 5.96 KB )
  136. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/contract/LogHandlerInterface.php ( 0.86 KB )
  137. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/log/Channel.php ( 3.89 KB )
  138. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/event/LogRecord.php ( 1.02 KB )
  139. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-helper/src/Collection.php ( 16.47 KB )
  140. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-orm/src/db/Raw.php ( 1.22 KB )
  141. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/View.php ( 1.70 KB )
  142. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/View.php ( 4.39 KB )
  143. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/view/driver/Php.php ( 6.44 KB )
  144. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/contract/TemplateHandlerInterface.php ( 1.60 KB )
  145. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/public.php ( 3.56 KB )
  146. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/public_param.php ( 0.75 KB )
  147. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/Request.php ( 9.20 KB )
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  149. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/Route.php ( 4.70 KB )
  150. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-multi-app/src/Url.php ( 7.52 KB )
  151. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/route/Url.php ( 14.07 KB )
  152. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/head.php ( 3.43 KB )
  153. /www/wwwroot/sbywcs.dsger.com/app/web/view/product/detail.php ( 31.31 KB )
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  1. CONNECT:[ UseTime:0.000512s ] mysql:host=127.0.0.1;port=3306;dbname=sbywcs_dsger_com;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000800s ]
  3. SELECT * FROM `goods` WHERE `code` = '61603' LIMIT 1 [ RunTime:0.000563s ]
  4. SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000530s ]
  5. SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000375s ]
  6. SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000411s ]
  7. SELECT * FROM `goods_album` WHERE `goods_id` = 7495 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000493s ]
  8. SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000528s ]
  9. SELECT * FROM `goods_quotation` WHERE `goods_id` = 7495 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000350s ]
  10. SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000552s ]
  11. SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000297s ]
  12. SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.055218s ]
  13. SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (5024,5538,10535,10166,10139,7302,4898,4166,8447,7335) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000891s ]
  14. SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000509s ]
  15. SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000229s ]
  16. SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000462s ]
  17. SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000256s ]
  18. SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000302s ]
  19. SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000408s ]
  20. SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000284s ]
  21. SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000252s ]
0.099267s