Western blotting analysis using cullin 2 antibody (Cat#61625). Total cell lysates (15 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cullin 2 antibody (Cat#61625, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using cullin 2 antibody (Cat#61625). Cullin 2 expression in wild-type (WT) and cullin 2 (CUL2) knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with cullin 2 antibody (Cat#61625, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Cullin 2 expression in HepG2 cells using Cullin 2 antibody (Cat#61625, 1:2,000). Green, isotype control; red, Cullin 2.
Immunocytochemical staining of HepG2 cells with cullin 2 antibody (Cat#61625, 1:1,000). Nuclei were stained blue with DAPI; Cullin 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
