Western blotting analysis using kinesin associated protein 3 antibody (Cat#61655). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with kinesin associated protein 3 antibody (Cat#61655, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using kinesin associated protein 3 antibody (Cat#61655). Kinesin associated protein 3 expression in wild type (WT) and kinesin associated protein 3 (KIFAP3) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with kinesin associated protein 3 antibody (Cat#61655, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Kinesin associated protein 3 expression in HepG2 cells using Kinesin associated protein 3 antibody (Cat#61655, 1:2,000). Green, isotype control; red, Kinesin associated protein 3.
KD-Validated Kinesin Associated Protein 3 Recombinant Rabbit mAb
Aliases
KIFAP3; Kinesin Associated Protein 3; SMAP; KAP3; Kinesin-Associated Protein 3; KAP-1; FLA3; Smg GDS-Associated Protein; KAP-3; Small G Protein GDP Dissociation Stimulator; DJ190I16.1; Smg GDS; Smg-GDS; KIF3AP
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
A synthesized peptide derived from human KIFAP3
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using kinesin associated protein 3 antibody (Cat#61655). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with kinesin associated protein 3 antibody (Cat#61655, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using kinesin associated protein 3 antibody (Cat#61655). Kinesin associated protein 3 expression in wild type (WT) and kinesin associated protein 3 (KIFAP3) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with kinesin associated protein 3 antibody (Cat#61655, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Kinesin associated protein 3 expression in HepG2 cells using Kinesin associated protein 3 antibody (Cat#61655, 1:2,000). Green, isotype control; red, Kinesin associated protein 3.
Western blotting analysis using kinesin associated protein 3 antibody (Cat#61655). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with kinesin associated protein 3 antibody (Cat#61655, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using kinesin associated protein 3 antibody (Cat#61655). Kinesin associated protein 3 expression in wild type (WT) and kinesin associated protein 3 (KIFAP3) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with kinesin associated protein 3 antibody (Cat#61655, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Kinesin associated protein 3 expression in HepG2 cells using Kinesin associated protein 3 antibody (Cat#61655, 1:2,000). Green, isotype control; red, Kinesin associated protein 3.
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