Western blotting analysis using Atg4C antibody (Cat#61741). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Atg4C antibody (Cat#61741,1:10,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201,1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using Atg4C antibody (Cat#61741). Atg4C expression in wild type (WT) and ATG4C shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Atg4C antibody (Cat#61741,1:10,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201,1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Atg4C antibody (Cat#61741, 1:1,000). Nuclei were stained blue with DAPI; Atg4C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Very low. Scale bar, 20 μm.
Immunocytochemical staining of HeLa cells using atg4C antibody (Cat#61741, 1:1,000), Top panel: wild-type (WT); Bottom panal: atg4C shRNA knockdown (KD). Nuclei were stained blue with DAPI; Atg4C was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Flow cytometric analysis of Atg4C expression in HepG2 cells using Atg4C antibody (Cat#61741,1:2,000). Green, isotype control; red, Atg4C.
Validation of Atg4C knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Atg4C antibody (Cat#61741, 1:2,000) and analyzed using BD flow cytometer.
