Western blotting analysis using prosaposin antibody (Cat#61752). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with prosaposin antibody (Cat#61752, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using prosaposin antibody (Cat#61752). Prosaposin expression in wild-type (WT) and prosaposin (PSAP) knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with prosaposin antibody (Cat#61752, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Prosaposin expression in HepG2 cells using Prosaposin antibody (Cat#61752, 1:2,000). Green, isotype control; red, Prosaposin.