Western blotting analysis using AKT3 antibody (Cat#61774). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with AKT3 antibody (Cat#61774, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using AKT3 antibody (Cat#61774). AKT3 expression in wild-type (WT) and AKT3 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with AKT3 antibody (Cat#61774, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of AKT3 expression in C2C12 cells using AKT3 antibody (Cat#61774, 1:2,000). Green, isotype control; red, AKT3.
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human AKT3
Isotype
Mouse IgG3
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:200-1:1,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using AKT3 antibody (Cat#61774). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with AKT3 antibody (Cat#61774, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using AKT3 antibody (Cat#61774). AKT3 expression in wild-type (WT) and AKT3 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with AKT3 antibody (Cat#61774, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of AKT3 expression in C2C12 cells using AKT3 antibody (Cat#61774, 1:2,000). Green, isotype control; red, AKT3.
Western blotting analysis using AKT3 antibody (Cat#61774). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with AKT3 antibody (Cat#61774, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using AKT3 antibody (Cat#61774). AKT3 expression in wild-type (WT) and AKT3 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with AKT3 antibody (Cat#61774, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of AKT3 expression in C2C12 cells using AKT3 antibody (Cat#61774, 1:2,000). Green, isotype control; red, AKT3.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000838s ]
SELECT * FROM `goods` WHERE `code` = '61774' LIMIT 1 [ RunTime:0.000620s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000599s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000451s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000544s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 7656 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000499s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000609s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 7656 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000408s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000551s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000390s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.058536s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (6353,4569,8588,6345,6557,6619,7633,9399,9999,5294) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000913s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000494s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000247s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000458s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000276s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000338s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000401s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000245s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000219s ]
