Western blotting analysis using HHEX antibody (Cat#61776). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HHEX antibody (Cat#61776, 1:10,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using HHEX antibody (Cat#61776). HHEX expression in wild type (WT) and HHEX shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with HHEX antibody (Cat#61776, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of HHEX expression in HepG2 cells using HHEX antibody (Cat#61776, 1:2,000). Green, isotype control; red, HHEX.
Immunocytochemical staining of HepG2 cells with HHEX antibody (Cat#61776, 1:1,000). Nuclei were stained blue with DAPI; HHEX was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Scale bar, 20 μm.
