Western blotting analysis using farnesyl-diphosphate farnesyltransferase 1 antibody (Cat#61778). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with farnesyl-diphosphate farnesyltransferase 1 antibody (Cat#61778, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using farnesyl-diphosphate farnesyltransferase 1 antibody (Cat#61778). Farnesyl-diphosphate farnesyltransferase 1 expression in wild type (WT) and farnesyl-diphosphate farnesyltransferase 1 (FDFT1) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with farnesyl-diphosphate farnesyltransferase 1 antibody (Cat#61778, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Farnesyl-diphosphate farnesyltransferase 1 expression in HAP-1 cells using Farnesyl-diphosphate farnesyltransferase 1 antibody (Cat#61778, 1:2,000). Green, isotype control; red, Farnesyl-diphosphate farnesyltransferase 1.