Western blotting analysis using mitogen-activated protein kinase 9 antibody (Cat#61790). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with mitogen-activated protein kinase 9 antibody (Cat#61790, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using mitogen-activated protein kinase 9 antibody (Cat#61790). Mitogen-activated protein kinase 9 expression in wild-type (WT) and mitogen-activated protein kinase 9 (MAPK9) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with mitogen-activated protein kinase 9 antibody (Cat#61790, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Mitogen-activated protein kinase 9 expression in HeLa cells using Mitogen-activated protein kinase 9 antibody (Cat#61790, 1:2,000). Green, isotype control; red, Mitogen-activated protein kinase 9.
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human MAPK9
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using mitogen-activated protein kinase 9 antibody (Cat#61790). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with mitogen-activated protein kinase 9 antibody (Cat#61790, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using mitogen-activated protein kinase 9 antibody (Cat#61790). Mitogen-activated protein kinase 9 expression in wild-type (WT) and mitogen-activated protein kinase 9 (MAPK9) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with mitogen-activated protein kinase 9 antibody (Cat#61790, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Mitogen-activated protein kinase 9 expression in HeLa cells using Mitogen-activated protein kinase 9 antibody (Cat#61790, 1:2,000). Green, isotype control; red, Mitogen-activated protein kinase 9.
Western blotting analysis using mitogen-activated protein kinase 9 antibody (Cat#61790). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with mitogen-activated protein kinase 9 antibody (Cat#61790, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using mitogen-activated protein kinase 9 antibody (Cat#61790). Mitogen-activated protein kinase 9 expression in wild-type (WT) and mitogen-activated protein kinase 9 (MAPK9) knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with mitogen-activated protein kinase 9 antibody (Cat#61790, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Mitogen-activated protein kinase 9 expression in HeLa cells using Mitogen-activated protein kinase 9 antibody (Cat#61790, 1:2,000). Green, isotype control; red, Mitogen-activated protein kinase 9.
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