Western blotting analysis using dihydropyrimidinase like 5 antibody (Cat#61833). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with dihydropyrimidinase like 5 antibody (Cat#61833, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using dihydropyrimidinase like 5 antibody (Cat#61833). Dihydropyrimidinase like 5 expression in wild-type (WT) and dihydropyrimidinase like 5 (DPYSL5) shRNA knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with dihydropyrimidinase like 5 antibody (Cat#61833, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of Dihydropyrimidinase like 5 expression in HAP-1 cells using Dihydropyrimidinase like 5 antibody (Cat#61833, 1:2,000). Green, isotype control; red, Dihydropyrimidinase like 5.
Validation of Dihydropyrimidinase like 5 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HSHC cells were stained with Dihydropyrimidinase like 5 antibody (Cat#61833, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HAP-1 cells with Dihydropyrimidinase like 5 antibody (Cat#61833, 1:1,000). Nuclei were stained blue with DAPI; Dihydropyrimidinase like 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunocytochemical staining of HAP-1 cells using Dihydropyrimidinase like 5 antibody (Cat#61833, 1:1,000), Top panel: wild-type (WT); Bottom panal: Dihydropyrimidinase like 5 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Dihydropyrimidinase like 5 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
