Western blotting analysis using HNRNPK antibody (Cat#61853). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with HNRNPK antibody (Cat#61853, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using HNRNPK antibody (Cat#61853). HNRNPK expression in wild type (WT) and HNRNPK shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with HNRNPK antibody (Cat#61853, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using HNRNPK antibody (Cat#61853, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse kidney using HNRNPK antibody (Cat#61853, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse brain using HNRNPK antibody (Cat#61853, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse liver using HNRNPK antibody (Cat#61853, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HepG2 cells with HNRNPK antibody (Cat #61853, 1:1,000) . Nuclei were stained blue with DAPI; HNRNPK was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunocytochemical staining of HeLa cells using HNRNPK antibody (Cat#61853, 1:1,000), Top panel: wild-type (WT); Bottom panal: HNRNPK shRNA knockdown (KD). Nuclei were stained blue with DAPI; HNRNPK was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Flow cytometric analysis of HNRNPK expression in HepG2 cells using HNRNPK antibody (Cat#61853, 1:2,000). Green, isotype control; red, HNRNPK.
