Western blotting analysis using Katanin catalytic subunit A1 antibody (Cat#61856). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Katanin catalytic subunit A1 antibody (Cat#61856, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using katanin catalytic subunit A1 antibody (Cat#61856). Katanin catalytic subunit A1 expression in wild type (WT) and katanin catalytic subunit A1 (KATNA1) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with katanin catalytic subunit A1 antibody (Cat#61856, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Hela cells with Katanin catalytic subunit A1 antibody (Cat#61856, 1:1,000). Nuclei were stained blue with DAPI; Katanin catalytic subunit A1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Immunocytochemical staining of HeLa cells using katanin catalytic subunit A1 antibody (Cat#61856, 1:1,000), Top panel: wild-type (WT); Bottom panal: KATNA1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Katanin catalytic subunit A1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Flow cytometric analysis of Katanin catalytic subunit A1 expression in HeLa cells using Katanin catalytic subunit A1 antibody (Cat#61856, 1:2,000). Green, isotype control; red, Katanin catalytic subunit A1.
Validation of Katanin catalytic subunit A1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Katanin catalytic subunit A1 antibody (Cat#61856, 1:2,000) and analyzed using BD flow cytometer.
