Western blotting analysis using Galectin 3 antibody (Cat#61857). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Galectin 3 antibody (Cat#61857, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using Galectin 3 antibody (Cat#61857). Galectin 3 expression in wild type (WT) and Galectin 3 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with Galectin 3 antibody (Cat#61857, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human papillary thyroid carcinoma using galectin 3 antibody (Cat#61857, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse spleen using galectin 3 antibody (Cat#61857, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Immunocytochemical staining of HepG2 cells with Galectin 3 antibody (Cat#61857, 1:1,000) . Nuclei were stained blue with DAPI; Galectin 3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of Galectin 3 expression in C2C12 cells using Galectin 3 antibody (Cat#61857, 1:2,000). Green, isotype control; red, Galectin 3.
