Western blotting analysis using RIGI antibody (Cat#61997). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with RIGI antibody (Cat#61997, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).
Western blotting analysis using RIGI antibody (Cat#61997). RIGI expression in wild-type (WT) and RIGI shRNA knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with RIGI antibody (Cat#61997, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of RIGI knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HAP1 cells were stained with RIGI antibody (Cat#61997, 1:2,000) and analyzed using BD flow cytometer.