Western blotting analysis using PIAS1 antibody (Cat#62002). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PIAS1 antibody (Cat#62002, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using PIAS1 antibody (Cat#62002). PIAS1 expression in wild-type (WT) and PIAS1 shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PIAS1 antibody (Cat#62002, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells using PIAS1 antibody (Cat#62002, 1:1,000), Top panel: wild-type (WT); Bottom panal: PIAS1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; PIAS1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen
A synthesized peptide derived from human PIAS1
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (ICC): 1:100-1:1,000
Note
This product is for research use only.
Data
Western blotting analysis using PIAS1 antibody (Cat#62002). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PIAS1 antibody (Cat#62002, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using PIAS1 antibody (Cat#62002). PIAS1 expression in wild-type (WT) and PIAS1 shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PIAS1 antibody (Cat#62002, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells using PIAS1 antibody (Cat#62002, 1:1,000), Top panel: wild-type (WT); Bottom panal: PIAS1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; PIAS1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Western blotting analysis using PIAS1 antibody (Cat#62002). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PIAS1 antibody (Cat#62002, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using PIAS1 antibody (Cat#62002). PIAS1 expression in wild-type (WT) and PIAS1 shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PIAS1 antibody (Cat#62002, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells using PIAS1 antibody (Cat#62002, 1:1,000), Top panel: wild-type (WT); Bottom panal: PIAS1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; PIAS1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
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