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  • Primary Antibody
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Home>Product>Primary Antibody

KD-Validated Integrin Subunit Beta 4 Recombinant Rabbit mAb

#62025

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shRNA Knockdown Validated
Western blotting analysis using integrin subunit beta 4 antibody (Cat#62025). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with integrin subunit beta 4 antibody (Cat#62025, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using integrin subunit beta 4 antibody (Cat#62025). Integrin subunit beta 4 expression in wild-type (WT) and integrin subunit beta 4 (ITGB4) shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with integrin subunit beta 4 antibody (Cat#62025, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of Integrin subunit beta 4 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HepG2 cells were stained with Integrin subunit beta 4 antibody (Cat#62025, 1:2,000) and analyzed using BD flow cytometer.
Please select the specifications of the product.
Specification：
20 μL 100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    ITGB4
  • Catalog：
    62025
  • Application：
    WB, FCM
  • Species Reactivity：
    H

Information

Data

Information
Product Name KD-Validated Integrin Subunit Beta 4 Recombinant Rabbit mAb
Aliases ITGB4; Integrin Subunit Beta 4; CD104; Integrin Beta-4; CD104 Antigen; GP150; Integrin, Beta 4; JEB5A; JEB5B
Background

Gene Name: ITGB4

NCBI Gene Entry: 3691

UniProt Entry: P16144

Application Information

Molecular Weight: Predicted, 202 kDa; observed, 180-220 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB7875

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen A synthesized peptide derived from human Integrin beta 4
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for 1 year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Note This product is for research use only.
Data
  • Western blotting analysis using integrin subunit beta 4 antibody (Cat#62025). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with integrin subunit beta 4 antibody (Cat#62025, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using integrin subunit beta 4 antibody (Cat#62025). Integrin subunit beta 4 expression in wild-type (WT) and integrin subunit beta 4 (ITGB4) shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with integrin subunit beta 4 antibody (Cat#62025, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Validation of Integrin subunit beta 4 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HepG2 cells were stained with Integrin subunit beta 4 antibody (Cat#62025, 1:2,000) and analyzed using BD flow cytometer.
Please select the specifications of the product.
Specification：
20 μL,100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    ITGB4
  • Catalog：
    62025
  • Application：
    WB, FCM
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using integrin subunit beta 4 antibody (Cat#62025). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with integrin subunit beta 4 antibody (Cat#62025, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using integrin subunit beta 4 antibody (Cat#62025). Integrin subunit beta 4 expression in wild-type (WT) and integrin subunit beta 4 (ITGB4) shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with integrin subunit beta 4 antibody (Cat#62025, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of Integrin subunit beta 4 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HepG2 cells were stained with Integrin subunit beta 4 antibody (Cat#62025, 1:2,000) and analyzed using BD flow cytometer.
Shopping cart (12)
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Estimated total order price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$23400
View the shopping cart Empty the shopping cart
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  • Email Address

    support@genuinbiotech.com

    sales@genuinbiotech.com

  • Office Hours

    8:30 AM - 5:30 PM EST Mon-Fri

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  150. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-multi-app/src/Url.php ( 7.52 KB )
  151. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/route/Url.php ( 14.07 KB )
  152. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/head.php ( 3.43 KB )
  153. /www/wwwroot/sbywcs.dsger.com/app/web/view/product/detail.php ( 31.31 KB )
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  1. CONNECT:[ UseTime:0.001215s ] mysql:host=127.0.0.1;port=3306;dbname=sbywcs_dsger_com;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `goods` [ RunTime:0.001823s ]
  3. SELECT * FROM `goods` WHERE `code` = '62025' LIMIT 1 [ RunTime:0.000710s ]
  4. SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.001002s ]
  5. SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001229s ]
  6. SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000790s ]
  7. SELECT * FROM `goods_album` WHERE `goods_id` = 9761 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000666s ]
  8. SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.001441s ]
  9. SELECT * FROM `goods_quotation` WHERE `goods_id` = 9761 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000843s ]
  10. SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.001002s ]
  11. SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000877s ]
  12. SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.134854s ]
  13. SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (4092,4191,4265,4540,5271,7654,5678,4377,6279,5415) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001637s ]
  14. SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000553s ]
  15. SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000205s ]
  16. SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000532s ]
  17. SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000247s ]
  18. SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.005252s ]
  19. SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000486s ]
  20. SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000291s ]
  21. SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000206s ]
0.216539s