Western blotting analysis using PIN1 antibody (Cat#62043). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PIN1 antibody (Cat#62043, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using PIN1 antibody (Cat#62043). PIN1 expression in wild-type (WT) and PIN1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PIN1 antibody (Cat#62043, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP1 cells with PIN1 antibody (Cat#62043,1:1,000). Nuclei were stained blue with DAPI; PIN1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of PIN1 expression in HAP-1 cells using PIN1 antibody (Cat#62043,1:2,000). Green, isotype control; red, PIN1.
Validation of PIN1 knockdown using flow cytometry. Wild-type(WT, Blue) and PIN1 knockdown(KD, Green) HeLa cells were stained with PIN1 antibody(Cat#62043, 1:2,000) and analyzed using BD flow cytometer.
