Western blotting analysis using STAT1 antibody (Cat#62066). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with STAT1 antibody (Cat#62066, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). STAT1, signal transducer and activator of transcription 1.
Western blotting analysis using STAT1 antibody (Cat#62066). STAT1 expression in wild type (WT) and STAT1 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with STAT1 antibody (Cat#62066, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
STAT1; Signal Transducer And Activator Of Transcription 1; Transcription Factor ISGF-3 Components P91/P84; STAT91; ISGF-3; Signal Transducer And Activator Of Transcription 1-Alpha/Beta; Signal Transducer And Activator Of Transcription 1, 91kDa; Signal Transducer And Activator Of Transcription 1, 91kD; CANDF7; IMD31A; IMD31B; IMD31C
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500
Note
This product is for research use only.
Data
Western blotting analysis using STAT1 antibody (Cat#62066). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with STAT1 antibody (Cat#62066, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). STAT1, signal transducer and activator of transcription 1.
Western blotting analysis using STAT1 antibody (Cat#62066). STAT1 expression in wild type (WT) and STAT1 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with STAT1 antibody (Cat#62066, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using STAT1 antibody (Cat#62066). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with STAT1 antibody (Cat#62066, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). STAT1, signal transducer and activator of transcription 1.
Western blotting analysis using STAT1 antibody (Cat#62066). STAT1 expression in wild type (WT) and STAT1 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with STAT1 antibody (Cat#62066, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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