Western blotting analysis using POLG antibody (Cat#62489). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with POLG antibody (Cat#62489, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using POLG antibody (Cat#62489). POLG expression in wild-type (WT) and POLG shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with POLG antibody (Cat#62489, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of POLG expression in HeLa cells using POLG antibody (Cat#62489, 1:2,000). Green, isotype control; red, POLG.
Immunocytochemical staining of HeLa cells with POLG antibody (Cat#62489, 1:1,000) . Nuclei were stained blue with DAPI; POLG was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium . Scale bar, 20 μm.
