Western blotting analysis using forkhead box o4 antibody (Cat#62585). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with forkhead box o4 antibody (Cat#62585, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using forkhead box o4 antibody (Cat#62585). Forkhead box o4 expression in wild type (WT) and forkhead box o4 ( FOXO4) shRNA knockdown (KD) 293T cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with forkhead box o4 antibody (Cat#62585, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded mouse liver using forkhead box o4 antibody (Cat#62585, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunocytochemical staining of HeLa cells with forkhead box o4 antibody (Cat#62585, 1:1,000). Nuclei were stained blue with DAPI; Forkhead box o4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunocytochemical staining of 293T cells using forkhead box o4 antibody (Cat#62585, 1:1,000), Top panel: wild-type (WT); Bottom panal: Forkhead box o4 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Forkhead box o4 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Flow cytometric analysis of Forkhead box O4 expression in HeLa cells using forkhead box o4 antibody (Cat#62585, 1:2,000). Green, isotype control; red, Forkhead box o4.
