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KD-Validated SLC25A12 Recombinant Rabbit mAb

#62662

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Recombinant Antibody
shRNA Knockdown Validated
Western blotting analysis using solute carrier family 25 member 12 antibody (Cat#62662). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with solute carrier family 25 member 12 antibody (Cat#62662, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using solute carrier family 25 member 12 antibody (Cat#62662). Solute carrier family 25 member 12 expression in wild type (WT) and solute carrier family 25 member 12 (SLC25A12) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with solute carrier family 25 member 12 antibody (Cat#62662, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with Solute carrier family 25 member 12 antibody (Cat#62662, 1:1,000) . Nuclei were stained blue with DAPI; Solute carrier family 25 member 12 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of Solute carrier family 25 member 12 expression in C2C12 cells using Solute carrier family 25 member 12 antibody (Cat#62662, 1:2,000). Green, isotype control; red, Solute carrier family 25 member 12.
Please select the specifications of the product.
Specification:
20 μL 100 μL
Unit price:
$190
Quantity:
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
  • Gene Symbol:
    SLC25A12
  • Catalog:
    62662
  • Application:
    WB, FCM, ICC
  • Species Reactivity:
    H, M, R
Information
Product Name KD-Validated SLC25A12 Recombinant Rabbit mAb
Aliases SLC25A12; Solute Carrier Family 25 Member 12; Aralar; AGC1; Electrogenic Aspartate/Glutamate Antiporter SLC25A12, Mitochondrial; Aralar1; Solute Carrier Family 25 (Mitochondrial Carrier, Aralar), Member 12; Solute Carrier Family 25 (Aspartate/Glutamate Carrier), Member 12; Mitochondrial Aspartate Glutamate Carrier 1; Aspartate/Glutamate Carrier 1; Araceli Hiperlarga; Calcium Binding Mitochondrial Carrier Superfamily Member Aralar1; Calcium-Binding Mitochondrial Carrier Protein Aralar1; ARALAR1; EIEE39; DEE39
Background

Gene Name: SLC25A12

NCBI Gene Entry: 8604

UniProt Entry: O75746

Application Information

Molecular Weight: Predicted, 75 kDa, observed, 72 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB2340

Species Reactivity: Human,mouse,rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)

Immunogen A synthesized peptide derived from human SLC25A12
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for 1 year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using solute carrier family 25 member 12 antibody (Cat#62662). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with solute carrier family 25 member 12 antibody (Cat#62662, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using solute carrier family 25 member 12 antibody (Cat#62662). Solute carrier family 25 member 12 expression in wild type (WT) and solute carrier family 25 member 12 (SLC25A12) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with solute carrier family 25 member 12 antibody (Cat#62662, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of C2C12 cells with Solute carrier family 25 member 12 antibody (Cat#62662, 1:1,000) . Nuclei were stained blue with DAPI; Solute carrier family 25 member 12 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Flow cytometric analysis of Solute carrier family 25 member 12 expression in C2C12 cells using Solute carrier family 25 member 12 antibody (Cat#62662, 1:2,000). Green, isotype control; red, Solute carrier family 25 member 12.
Western blotting analysis using solute carrier family 25 member 12 antibody (Cat#62662). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with solute carrier family 25 member 12 antibody (Cat#62662, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using solute carrier family 25 member 12 antibody (Cat#62662). Solute carrier family 25 member 12 expression in wild type (WT) and solute carrier family 25 member 12 (SLC25A12) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with solute carrier family 25 member 12 antibody (Cat#62662, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of C2C12 cells with Solute carrier family 25 member 12 antibody (Cat#62662, 1:1,000) . Nuclei were stained blue with DAPI; Solute carrier family 25 member 12 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of Solute carrier family 25 member 12 expression in C2C12 cells using Solute carrier family 25 member 12 antibody (Cat#62662, 1:2,000). Green, isotype control; red, Solute carrier family 25 member 12.
  • Phone Number

    (540)855-7041

  • Email Address

    support@genuinbiotech.com

    sales@genuinbiotech.com

  • Office Hours

    8:30 AM - 5:30 PM EST Mon-Fri

微信公众号

Copyright © 2025 Hefei GenuIN Biotech Co., Ltd.

All Rights Reserved.

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  147. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/Request.php ( 9.20 KB )
  148. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/mobile.php ( 4.08 KB )
  149. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/Route.php ( 4.70 KB )
  150. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-multi-app/src/Url.php ( 7.52 KB )
  151. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/route/Url.php ( 14.07 KB )
  152. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/head.php ( 3.43 KB )
  153. /www/wwwroot/sbywcs.dsger.com/app/web/view/product/detail.php ( 31.31 KB )
  154. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/public_cart.php ( 3.88 KB )
  155. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/foot.php ( 5.05 KB )
  156. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/Response.php ( 8.81 KB )
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  158. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-trace/src/Html.php ( 4.42 KB )
  1. CONNECT:[ UseTime:0.000476s ] mysql:host=127.0.0.1;port=3306;dbname=sbywcs_dsger_com;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000767s ]
  3. SELECT * FROM `goods` WHERE `code` = '62662' LIMIT 1 [ RunTime:0.000504s ]
  4. SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000460s ]
  5. SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000344s ]
  6. SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000419s ]
  7. SELECT * FROM `goods_album` WHERE `goods_id` = 8040 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000443s ]
  8. SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000426s ]
  9. SELECT * FROM `goods_quotation` WHERE `goods_id` = 8040 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000293s ]
  10. SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000393s ]
  11. SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000281s ]
  12. SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.065192s ]
  13. SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (4611,7919,4188,8656,8784,6170,9074,5801,4920,8516) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000866s ]
  14. SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000476s ]
  15. SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000241s ]
  16. SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000492s ]
  17. SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000267s ]
  18. SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000303s ]
  19. SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000406s ]
  20. SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000262s ]
  21. SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000277s ]
0.104104s