Western blotting analysis using Tropomyosin 1 antibody (Cat#62674). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with Tropomyosin 1 antibody (Cat#62674, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using Tropomyosin 1 antibody (Cat#62674). Tropomyosin 1 expression in wild type (WT) and Tropomyosin 1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with Tropomyosin 1 antibody (Cat#62674, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Tropomyosin 1 expression in HepG2 cells using Tropomyosin 1 antibody (Cat#62674, 1:2,000). Green, isotype control; red, Tropomyosin 1.