Western blotting analysis using lymphocyte antigen 75 antibody (Cat#6303). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with lymphocyte antigen 75 antibody (Cat#6303, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of Raji cells with lymphocyte antigen 75 antibody (Cat#6303, 1:1,000). Nuclei were stained blue with DAPI; Lymphocyte antigen 75 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Flow cytometric analysis of lymphocyte antigen 75 expression in Raji cells using lymphocyte antigen 75 antibody (Cat#6303, 1:2,000). Green, isotype control; red, Lymphocyte antigen 75.