Western blotting analysis using phospho-PKC alpha (Thr638) antibody (Cat#63196). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with phospho-PKC alpha (Thr638) antibody (Cat#63196, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using phospho-PKC alpha (Thr638) antibody (Cat#63196). Phospho-PKC alpha (Thr638) expression in wild type (WT) and PRKCA shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with phospho-PKC alpha (Thr638) antibody (Cat#63196, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of phospho-PKC alpha (Thr638) expression in HepG2 cells using phospho-PKC alpha (Thr638) antibody (Cat#63196, 1:2,000). Green, isotype control; red, phospho-PKC alpha (Thr638).
Immunocytochemical staining of HepG2 cells with phospho-PKC alpha (Thr638) antibody (Cat#63196, 1:1,000). Nuclei were stained blue with DAPI; Phospho-PKC alpha (Thr638) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
