Western blotting analysis using cystathionine gamma-lyase antibody (Cat#63268). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with cystathionine gamma-lyase antibody (Cat#63268, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using cystathionine gamma-lyase antibody (Cat#63268). Cystathionine gamma-lyase expression in wild type (WT) and cystathionine gamma-lyase (CTH) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with cystathionine gamma-lyase antibody (Cat#63268, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of cystathionine gamma-lyase expression in HepG2 cells using cystathionine gamma-lyase antibody (Cat#63268, 1:2,000). Green, isotype control; red, Cystathionine Gamma-Lyase.