Western blotting analysis using epidermal growth factor receptor antibody (Cat#63456). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with epidermal growth factor receptor antibody (Cat#63456, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
Western blotting analysis using epidermal growth factor receptor antibody (Cat#63456). Epidermal growth factor receptor expression in wild type (WT) and epidermal growth factor receptor (EGFR) shRNA knockdown (KD) HepG2 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with epidermal growth factor receptor antibody (Cat#63456, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HepG2 cells with Epidermal growth factor receptor antibody (Cat#63456, 1:1,000) . Nuclei were stained blue with DAPI; Epidermal growth factor receptor was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Low. Scale bar, 20 μm.
Immunocytochemical staining of HepG2 cells using Epidermal growth factor receptor antibody (Cat#63456, 1:1,000), Top panel: wild-type (WT); Bottom panal: Adiponectin receptor 1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Epidermal growth factor receptor was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
Western blotting analysis using anti-epidermal growth factor receptor antibody (Cat #63456). Total cell lysates (20 ug) from various breast cancer cell lines were loaded and separated by SDS-PAGE. The membrane was incubated with the respective primary antibody and corresponding HRP-conjugated secondary antibody. Images were developed using Clarity Western ECL Substrate. For reference, we use the Bio-Rad Dual Protein Ladder and the provided ladder images are merged colorimetric. Included for each image is expected observed protein sizes, the antibody dilution, and chemiluminescent exposure time.
