Western blotting analysis using MICA antibody (Cat#6354). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MICA antibody (Cat#6354, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of MICA expression in HaCaT cells using MICA antibody (Cat#6354,1:2,000). Green, isotype control; red, MICA.
MICA; MHC Class I Polypeptide-Related Sequence A; PERB11.1; MIC-A; Major Histocompatibility Complex Class I Chain-Related Protein A; MHC Class I Chain-Related Protein A; Stress Inducible Class I Homolog; MHC Class I Related Sequence A; MHC Class I Related Chain A; HLA Class I Antigen
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human MICA
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:400-1:2,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using MICA antibody (Cat#6354). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MICA antibody (Cat#6354, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of MICA expression in HaCaT cells using MICA antibody (Cat#6354,1:2,000). Green, isotype control; red, MICA.
Western blotting analysis using MICA antibody (Cat#6354). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MICA antibody (Cat#6354, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Flow cytometric analysis of MICA expression in HaCaT cells using MICA antibody (Cat#6354,1:2,000). Green, isotype control; red, MICA.
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