Western blotting analysis using APMAP antibody (Cat#63542). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with APMAP antibody (Cat#63542, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using APMAP antibody (Cat#63542). APMAP expression in wild type (WT) and APMAP shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with APMAP antibody (Cat#63542, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of APMAP expression in HT-1080 cells using APMAP antibody (Cat#63542, 1:2,000). Green, isotype control; red, APMAP.
Immunocytochemical staining of HT-1080 cells with APMAP antibody (Cat#63542, 1:1,000) . Nuclei were stained blue with DAPI; APMAP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
