Western blotting analysis using granulin precursor antibody (Cat#63575). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with granulin precursor antibody (Cat#63575, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using granulin precursor antibody (Cat#63575). Granulin precursor expression in wild type (WT) and granulin precursor (GRN) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with granulin precursor antibody (Cat#63575, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with granulin precursor antibody (Cat#63575, 1:1,000) . Nuclei were stained blue with DAPI; Granulin precursor was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Low. Scale bar, 20 μm.
Immunocytochemical staining of HeLa cells using Granulin precursor antibody (Cat#63575, 1:1,000), Top panel: wild-type (WT); Bottom panal: Granulin precursor shRNA knockdown (KD). Nuclei were stained blue with DAPI; Granulin precursor was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Flow cytometric analysis of granulin precursor expression in HepG2 cells using Granulin precursor antibody (Cat#63575, 1:2,000). Green, isotype control; red, Granulin precursor.
Validation of granulin precursor knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with granulin precursor antibody (Cat#63575, 1:2,000) and analyzed using BD flow cytometer.
