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  • Primary Antibody
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Home>Product>Primary Antibody

WT1 Mouse mAb

#6358

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Western blotting analysis using WT1 antibody (Cat#6358). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with WT1 antibody (Cat#6358, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with WT1 antibody (Cat#6358, 1:1,000) . Nuclei were stained blue with DAPI; WT1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Flow cytometric analysis of WT1 expression in HAP-1 cells using WT1 antibody (Cat#6358,1:2,000). Green, isotype control; red, WT1.
Please select the specifications of the product.
Specification：
20 μL 100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    WT1
  • Catalog：
    6358
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name WT1 Mouse mAb
Aliases WT1; WT1 Transcription Factor; WIT-2; NPHS4; WAGR; AWT1; WT-1; Wilms Tumor Protein; Wilms Tumor 1; WT33; GUD
Background

Gene Name: WT1

NCBI Gene Entry: 7490

UniProt Entry: P19544

Application Information

Molecular Weight: Predicted, 49 kDa; observed, 48-65 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB8665

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human WT1
Isotype Mouse IgG2a
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for 1 year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using WT1 antibody (Cat#6358). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with WT1 antibody (Cat#6358, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HAP-1 cells with WT1 antibody (Cat#6358, 1:1,000) . Nuclei were stained blue with DAPI; WT1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
  • Flow cytometric analysis of WT1 expression in HAP-1 cells using WT1 antibody (Cat#6358,1:2,000). Green, isotype control; red, WT1.
Please select the specifications of the product.
Specification：
20 μL,100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    WT1
  • Catalog：
    6358
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using WT1 antibody (Cat#6358). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with WT1 antibody (Cat#6358, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HAP-1 cells with WT1 antibody (Cat#6358, 1:1,000) . Nuclei were stained blue with DAPI; WT1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Flow cytometric analysis of WT1 expression in HAP-1 cells using WT1 antibody (Cat#6358,1:2,000). Green, isotype control; red, WT1.
Shopping cart (12)
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Estimated total order price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$23400
View the shopping cart Empty the shopping cart
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  154. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/public_cart.php ( 3.88 KB )
  155. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/foot.php ( 5.05 KB )
  156. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/Response.php ( 8.81 KB )
  157. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/response/Html.php ( 0.97 KB )
  158. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-trace/src/Html.php ( 4.42 KB )
  1. CONNECT:[ UseTime:0.005138s ] mysql:host=127.0.0.1;port=3306;dbname=sbywcs_dsger_com;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `goods` [ RunTime:0.001574s ]
  3. SELECT * FROM `goods` WHERE `code` = '6358' LIMIT 1 [ RunTime:0.002573s ]
  4. SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000490s ]
  5. SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000351s ]
  6. SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.002686s ]
  7. SELECT * FROM `goods_album` WHERE `goods_id` = 10176 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.002407s ]
  8. SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000511s ]
  9. SELECT * FROM `goods_quotation` WHERE `goods_id` = 10176 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.002007s ]
  10. SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000457s ]
  11. SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000269s ]
  12. SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.217874s ]
  13. SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (3891,5907,8553,9618,6057,8148,4961,7399,6009,6697) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000866s ]
  14. SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000394s ]
  15. SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000745s ]
  16. SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.001280s ]
  17. SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000987s ]
  18. SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000997s ]
  19. SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.004597s ]
  20. SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.003508s ]
  21. SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.002666s ]
0.308460s