Western blotting analysis using GIPC1 antibody (Cat#63585). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with GIPC1 antibody (Cat#63585, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using GIPC1 antibody (Cat#63585). GIPC1 expression in wild type (WT) and GIPC1 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with GIPC1 antibody (Cat#63585, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with GIPC1 antibody (Cat#63585, 1:1,000) . Nuclei were stained blue with DAPI; GIPC1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Scale bar, 20 μm.
Immunocytochemical staining of HT-1080 cells using GIPC1 antibody (Cat#63585, 1:1,000), Top panel: wild-type (WT); Bottom panal: GIPC1 shRNA knockdown (KD). Nuclei were stained blue with DAPI;GIPC1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
Flow cytometric analysis of GIPC1 expression in HepG2 cells using GIPC1 antibody (Cat#63585, 1:2,000). Green, isotype control; red, GIPC1.
