Western blotting analysis using annexin A1 antibody (Cat#63702). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with annexin A1 antibody (Cat#63702, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using annexin A1 antibody (Cat#63702). Annexin A1 expression in wild-type (WT) and annexin A1 (ANXA1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with annexin A1 antibody (Cat#63702, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of Annexin A1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Annexin A1 antibody (Cat#63702, 1:2,000) and analyzed using BD flow cytometer.
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human Annexin A1 beta
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using annexin A1 antibody (Cat#63702). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with annexin A1 antibody (Cat#63702, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using annexin A1 antibody (Cat#63702). Annexin A1 expression in wild-type (WT) and annexin A1 (ANXA1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with annexin A1 antibody (Cat#63702, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of Annexin A1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Annexin A1 antibody (Cat#63702, 1:2,000) and analyzed using BD flow cytometer.
Western blotting analysis using annexin A1 antibody (Cat#63702). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with annexin A1 antibody (Cat#63702, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using annexin A1 antibody (Cat#63702). Annexin A1 expression in wild-type (WT) and annexin A1 (ANXA1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with annexin A1 antibody (Cat#63702, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of Annexin A1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Annexin A1 antibody (Cat#63702, 1:2,000) and analyzed using BD flow cytometer.
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