Western blotting analysis using YY1 antibody (Cat#63709). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with YY1 antibody (Cat#63709, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using YY1 antibody (Cat#63709). YY1 expression in wild-type (WT) and YY1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with YY1 antibody (Cat#63709, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of YY1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with YY1 antibody (Cat#63709, 1:2,000) and analyzed using BD flow cytometer.
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human YY1
Isotype
Mouse IgG2a
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using YY1 antibody (Cat#63709). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with YY1 antibody (Cat#63709, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using YY1 antibody (Cat#63709). YY1 expression in wild-type (WT) and YY1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with YY1 antibody (Cat#63709, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of YY1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with YY1 antibody (Cat#63709, 1:2,000) and analyzed using BD flow cytometer.
Western blotting analysis using YY1 antibody (Cat#63709). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with YY1 antibody (Cat#63709, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using YY1 antibody (Cat#63709). YY1 expression in wild-type (WT) and YY1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with YY1 antibody (Cat#63709, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of YY1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with YY1 antibody (Cat#63709, 1:2,000) and analyzed using BD flow cytometer.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000660s ]
SELECT * FROM `goods` WHERE `code` = '63709' LIMIT 1 [ RunTime:0.000440s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000452s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000320s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000355s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 8303 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000361s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.003090s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 8303 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000290s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000396s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000261s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.088479s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (5922,70,8292,6496,9416,9617,4050,7529,5667,5943) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000870s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000458s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000228s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000516s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000230s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000341s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000377s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000265s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000199s ]
