Western blotting analysis using MAPK3 antibody (Cat#63710). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MAPK3 antibody (Cat#63710, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using MAPK3 antibody (Cat#63710). MAPK3 expression in wild-type (WT) and MAPK3 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with MAPK3 antibody (Cat#63710, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of MAPK3 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with MAPK3 antibody (Cat#63710, 1:2,000) and analyzed using BD flow cytometer.
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
A synthesized peptide derived from human ERK1
Isotype
Mouse IgG1 kappa
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:200-1:2,000
Note
This product is for research use only.
Data
Western blotting analysis using MAPK3 antibody (Cat#63710). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MAPK3 antibody (Cat#63710, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using MAPK3 antibody (Cat#63710). MAPK3 expression in wild-type (WT) and MAPK3 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with MAPK3 antibody (Cat#63710, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of MAPK3 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with MAPK3 antibody (Cat#63710, 1:2,000) and analyzed using BD flow cytometer.
Western blotting analysis using MAPK3 antibody (Cat#63710). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MAPK3 antibody (Cat#63710, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using MAPK3 antibody (Cat#63710). MAPK3 expression in wild-type (WT) and MAPK3 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with MAPK3 antibody (Cat#63710, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of MAPK3 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with MAPK3 antibody (Cat#63710, 1:2,000) and analyzed using BD flow cytometer.
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000992s ]
SELECT * FROM `goods` WHERE `code` = '63710' LIMIT 1 [ RunTime:0.000856s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.001244s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000645s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000403s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 8304 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000303s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000680s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 8304 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000474s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000759s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000344s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.077754s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (5459,9596,10,7241,6445,7465,8113,6655,8410,3788) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.004411s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000477s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000216s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.005188s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.001415s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000312s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.002474s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000262s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.004432s ]
