Western blotting analysis using ATP5C1 antibody (Cat#63811). Total cell lysates (10 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with ATP5C1 antibody (Cat#63811, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using ATP5C1 antibody (Cat#63811). ATP5C1 expression in wild type (WT) and ATP5C1(ATP5F1C) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with ATP5C1 antibody (Cat#63811, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HepG2 cells with ATP5C1 antibody (Cat#63811, 1:500). Nuclei were stained blue with DAPI; ATP5C1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunocytochemical staining of HT-1080 cells using ATP5C1 antibody (Cat#63811, 1:1,000), Top panel: wild-type (WT); Bottom panal: ATP5C1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; ATP5C1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Flow cytometric analysis of ATP5C1 expression in HepG2 cells using ATP5C1 antibody (Cat#63811, 1:1,000). Green, isotype control; red, ATP5C1.
Validation of ATP5C1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HT-1080 cells were stained with ATP5C1 antibody (Cat#63811, 1:2,000) and analyzed using BD flow cytometer.
