Western blotting analysis using FBXO21 antibody (Cat#63846). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FBXO21 antibody (Cat#63846, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using FBXO21 antibody (Cat#63846). FBXO21 expression in wild type (WT) and FBXO21 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with FBXO21 antibody (Cat#63846, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of FBXO21 expression in HAP-1 cells using FBXO21 antibody (Cat#63846, 1:1,000). Green, isotype control; red, FBXO21.
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human FBXO21
Isotype
Mouse IgG2a
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:100-1:1,000
Note
This product is for research use only.
Data
Western blotting analysis using FBXO21 antibody (Cat#63846). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FBXO21 antibody (Cat#63846, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using FBXO21 antibody (Cat#63846). FBXO21 expression in wild type (WT) and FBXO21 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with FBXO21 antibody (Cat#63846, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of FBXO21 expression in HAP-1 cells using FBXO21 antibody (Cat#63846, 1:1,000). Green, isotype control; red, FBXO21.
Western blotting analysis using FBXO21 antibody (Cat#63846). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with FBXO21 antibody (Cat#63846, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using FBXO21 antibody (Cat#63846). FBXO21 expression in wild type (WT) and FBXO21 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with FBXO21 antibody (Cat#63846, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of FBXO21 expression in HAP-1 cells using FBXO21 antibody (Cat#63846, 1:1,000). Green, isotype control; red, FBXO21.
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