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  • Primary Antibody
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Home>Product>Primary Antibody

Keratin 8 Mouse mAb

#6482

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Western blotting analysis using keratin 8 antibody (Cat#6482). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with keratin 8 antibody (Cat#6482, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HACAT cells with keratin 8 antibody (Cat#6482, 1:1,000). Nuclei were stained blue with DAPI; Keratin 8 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Flow cytometric analysis of Keratin 8 expression in HaCaT cells using Keratin 8 antibody (Cat#6482, 1:2,000). Green, isotype control; red, Keratin 8.
Please select the specifications of the product.
Specification：
20 μL，100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    KRT8
  • Catalog：
    6482
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H

Information

Data

Information
Product Name Keratin 8 Mouse mAb
Aliases KRT8; Keratin 8; CK-8; CYK8; K8; CARD2; K2C8; CK8; KO; Keratin, Type II Cytoskeletal 8; Type-II Keratin Kb8; Keratin 8, Type II; Cytokeratin-8; Keratin-8
Background

Gene Name: KRT8

NCBI Gene Entry: 3856

UniProt Entry: P05787

Application Information

Molecular Weight: Predicted, 54 kDa; observed, 50 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 25GB9520

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (ICC)
Immunogen A synthesized peptide derived from human KRT8
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for 1 year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Flow Cytometry (FCM): 1:200-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using keratin 8 antibody (Cat#6482). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with keratin 8 antibody (Cat#6482, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Immunocytochemical staining of HACAT cells with keratin 8 antibody (Cat#6482, 1:1,000). Nuclei were stained blue with DAPI; Keratin 8 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
  • Flow cytometric analysis of Keratin 8 expression in HaCaT cells using Keratin 8 antibody (Cat#6482, 1:2,000). Green, isotype control; red, Keratin 8.
Please select the specifications of the product.
Specification：
20 μL 100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    KRT8
  • Catalog：
    6482
  • Application：
    WB, FCM, ICC
  • Species Reactivity：
    H
RELATED PRODUCTS
Western blotting analysis using keratin 8 antibody (Cat#6482). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with keratin 8 antibody (Cat#6482, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunocytochemical staining of HACAT cells with keratin 8 antibody (Cat#6482, 1:1,000). Nuclei were stained blue with DAPI; Keratin 8 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Flow cytometric analysis of Keratin 8 expression in HaCaT cells using Keratin 8 antibody (Cat#6482, 1:2,000). Green, isotype control; red, Keratin 8.
Shopping cart (12)
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Estimated total order price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$23400
View the shopping cart Empty the shopping cart
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  153. /www/wwwroot/sbywcs.dsger.com/app/web/view/product/detail.php ( 31.31 KB )
  154. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/public_cart.php ( 3.88 KB )
  155. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/foot.php ( 5.05 KB )
  156. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/Response.php ( 8.81 KB )
  157. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/response/Html.php ( 0.97 KB )
  158. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-trace/src/Html.php ( 4.42 KB )
  1. CONNECT:[ UseTime:0.000483s ] mysql:host=127.0.0.1;port=3306;dbname=sbywcs_dsger_com;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000789s ]
  3. SELECT * FROM `goods` WHERE `code` = '6482' LIMIT 1 [ RunTime:0.000566s ]
  4. SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000523s ]
  5. SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000444s ]
  6. SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000495s ]
  7. SELECT * FROM `goods_album` WHERE `goods_id` = 10328 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000370s ]
  8. SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000485s ]
  9. SELECT * FROM `goods_quotation` WHERE `goods_id` = 10328 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000454s ]
  10. SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000599s ]
  11. SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000358s ]
  12. SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.064440s ]
  13. SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (8441,7147,3962,4877,4323,8840,7323,8959,4832,10165) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001054s ]
  14. SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000536s ]
  15. SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000251s ]
  16. SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000489s ]
  17. SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000283s ]
  18. SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000307s ]
  19. SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000404s ]
  20. SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000247s ]
  21. SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000223s ]
0.104392s