Western blotting analysis using SNX9 antibody (Cat#64838). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SNX9 antibody (Cat#64838, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using SNX9 antibody (Cat #64838). SNX9 expression in wild-type (WT) and SNX9 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SNX9 antibody (Cat #64838, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat #101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat #226).
Immunocytochemical staining of Hela cells with SNX9 antibody (Cat#64838, 1:1,000). Nuclei were stained blue with DAPI; SNX9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Immunocytochemical staining of HeLa cells using SNX9 antibody (Cat#64838, 1:1,000), Top panel: wild-type (WT); Bottom panal: SNX9 shRNA knockdown (KD). Nuclei were stained blue with DAPI; SNX9 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Flow cytometric analysis of SNX9 expression in HeLa cells using SNX9 antibody (Cat#64838, 1:2,000). Green, isotype control; red, SNX9.
Validation of SNX9 knockdown using flow cytometry. Wild-type(WT, Blue) and SNX9 knockdown(KD, Green) HeLa cells were stained with SNX9 antibody(Cat#64838, 1:2,000) and analyzed using BD flow cytometer.
