Western blotting analysis using NMNAT1 antibody (Cat#64842). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NMNAT1 antibody (Cat#64842, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using NMNAT1 antibody (Cat #64842). NMNAT1 expression in wild-type (WT) and NMNAT1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NMNAT1 antibody (Cat #64842, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat #101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat #716).
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500
Note
This product is for research use only.
Data
Western blotting analysis using NMNAT1 antibody (Cat#64842). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NMNAT1 antibody (Cat#64842, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using NMNAT1 antibody (Cat #64842). NMNAT1 expression in wild-type (WT) and NMNAT1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NMNAT1 antibody (Cat #64842, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat #101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat #716).
Western blotting analysis using NMNAT1 antibody (Cat#64842). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NMNAT1 antibody (Cat#64842, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using NMNAT1 antibody (Cat #64842). NMNAT1 expression in wild-type (WT) and NMNAT1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NMNAT1 antibody (Cat #64842, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat #101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat #716).
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.001351s ]
SELECT * FROM `goods` WHERE `code` = '64842' LIMIT 1 [ RunTime:0.000518s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.002702s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000325s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000368s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 8393 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000326s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.002518s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 8393 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000260s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000478s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000245s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.111116s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (7700,6652,8644,6018,6384,4643,10557,285,4533,9298) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.001816s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000466s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.003348s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000481s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000222s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000323s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000370s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000252s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000248s ]
