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  • Primary Antibody
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Home>Product>Primary Antibody

KD-Validated NAE1 Mouse mAb

#64857

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shRNA Knockdown Validated
Western blotting analysis using NAE1 antibody (Cat#64857). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NAE1 antibody (Cat#64857, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using NAE1 antibody (Cat#64857). NAE1 expression in wild-type (WT) and NAE1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NAE1 antibody (Cat#64857, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HeLa cells using NAE1 antibody (Cat#64857, 1:1,000), Top panel: wild-type (WT); Bottom panal: NAE1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; NAE1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Please select the specifications of the product.
Specification：
20 μL 100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    NAE1
  • Catalog：
    64857
  • Application：
    WB, ICC
  • Species Reactivity：
    H, R

Information

Data

Information
Product Name KD-Validated NAE1 Mouse mAb
Aliases NAE1; NEDD8 Activating Enzyme E1 Subunit 1; APP-BP1; APPBP1; Ula-1; Amyloid Beta Precursor Protein-Binding Protein 1, 59 KDa ; Amyloid Beta Precursor Protein Binding Protein 1, 59kDa; NEDD8-Activating Enzyme E1 Regulatory Subunit; Amyloid Protein-Binding Protein 1; Proto-Oncogene Protein 1; Amyloid Beta Precursor Protein-Binding Protein 1, 59kD; NEDD8-Activating Enzyme E1 Subunit; Protooncogene Protein 1; A-116A10.1; NEDFIH; HPP1
Background

Gene Name: NAE1

NCBI Gene Entry: 8883

UniProt Entry: Q13564

Application Information

Molecular Weight: Predicted, 60 kDa, observed, 60 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB13290

Species Reactivity: Human,rat

Applications Tested: Western blotting (WB), immunocytochemistry (ICC)
Immunogen Recombinant protein of human NAE1
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for 1 year.
Recommended Dilutions Western Blotting (WB): 1:400-1:2,000
Immunocytochemistry (ICC): 1:100-1:1,000
Note This product is for research use only.
Data
  • Western blotting analysis using NAE1 antibody (Cat#64857). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NAE1 antibody (Cat#64857, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using NAE1 antibody (Cat#64857). NAE1 expression in wild-type (WT) and NAE1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NAE1 antibody (Cat#64857, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunocytochemical staining of HeLa cells using NAE1 antibody (Cat#64857, 1:1,000), Top panel: wild-type (WT); Bottom panal: NAE1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; NAE1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Please select the specifications of the product.
Specification：
20 μL,100 μL
Unit price：
$190
Quantity：
1
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Add to Cart
  • Gene Symbol：
    NAE1
  • Catalog：
    64857
  • Application：
    WB, ICC
  • Species Reactivity：
    H, R
RELATED PRODUCTS
Western blotting analysis using NAE1 antibody (Cat#64857). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with NAE1 antibody (Cat#64857, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using NAE1 antibody (Cat#64857). NAE1 expression in wild-type (WT) and NAE1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with NAE1 antibody (Cat#64857, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HeLa cells using NAE1 antibody (Cat#64857, 1:1,000), Top panel: wild-type (WT); Bottom panal: NAE1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; NAE1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Shopping cart (12)
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
#R0001
KD-Validated Phospho-Tau (T181) Rabbit mAb
Knockdown Validated
Specification：
20 μL 50 μL 100 μL
Unit price：
$190
Quantity：
125
$950(Excluding sales tax)
In stock. If you place the order before 15:00 today, it will be dispatched today.
Estimated total order price
The final total price including taxes and shipping fees will be calculated at the checkout stage.
Subtotal：$23400
View the shopping cart Empty the shopping cart
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  • Email Address

    support@genuinbiotech.com

    sales@genuinbiotech.com

  • Office Hours

    8:30 AM - 5:30 PM EST Mon-Fri

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  146. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/public_param.php ( 0.75 KB )
  147. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/Request.php ( 9.20 KB )
  148. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/mobile.php ( 4.08 KB )
  149. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/facade/Route.php ( 4.70 KB )
  150. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-multi-app/src/Url.php ( 7.52 KB )
  151. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/route/Url.php ( 14.07 KB )
  152. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/head.php ( 3.43 KB )
  153. /www/wwwroot/sbywcs.dsger.com/app/web/view/product/detail.php ( 31.31 KB )
  154. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/public_cart.php ( 3.88 KB )
  155. /www/wwwroot/sbywcs.dsger.com/app/web/view/public/foot.php ( 5.05 KB )
  156. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/Response.php ( 8.81 KB )
  157. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/framework/src/think/response/Html.php ( 0.97 KB )
  158. /www/wwwroot/sbywcs.dsger.com/vendor/topthink/think-trace/src/Html.php ( 4.42 KB )
  1. CONNECT:[ UseTime:0.000556s ] mysql:host=127.0.0.1;port=3306;dbname=sbywcs_dsger_com;charset=utf8mb4
  2. SHOW FULL COLUMNS FROM `goods` [ RunTime:0.002311s ]
  3. SELECT * FROM `goods` WHERE `code` = '64857' LIMIT 1 [ RunTime:0.000699s ]
  4. SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000664s ]
  5. SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000444s ]
  6. SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000514s ]
  7. SELECT * FROM `goods_album` WHERE `goods_id` = 8402 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000472s ]
  8. SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000563s ]
  9. SELECT * FROM `goods_quotation` WHERE `goods_id` = 8402 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000342s ]
  10. SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000743s ]
  11. SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000357s ]
  12. SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.084407s ]
  13. SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (5347,8526,7353,9050,6884,6401,8453,8130,10163,4041) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000787s ]
  14. SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000470s ]
  15. SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000208s ]
  16. SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000519s ]
  17. SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000234s ]
  18. SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000325s ]
  19. SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000391s ]
  20. SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000234s ]
  21. SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000247s ]
0.126211s