Western blotting analysis using paxillin antibody (Cat#64968). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with paxillin antibody (Cat#64968, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using paxillin antibody (Cat#64968). Paxillin expression in wild-type (WT) and paxillin (PXN) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with paxillin antibody (Cat#64968, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500
Note
This product is for research use only.
Data
Western blotting analysis using paxillin antibody (Cat#64968). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with paxillin antibody (Cat#64968, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using paxillin antibody (Cat#64968). Paxillin expression in wild-type (WT) and paxillin (PXN) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with paxillin antibody (Cat#64968, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using paxillin antibody (Cat#64968). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with paxillin antibody (Cat#64968, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using paxillin antibody (Cat#64968). Paxillin expression in wild-type (WT) and paxillin (PXN) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with paxillin antibody (Cat#64968, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.001283s ]
SELECT * FROM `goods` WHERE `code` = '64968' LIMIT 1 [ RunTime:0.001048s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000749s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000945s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.001292s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 8446 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000472s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.001241s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 8446 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000683s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000762s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000820s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.172957s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (7392,10057,8188,7936,7024,10489,7146,7876,7605,9305) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000872s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.002576s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000244s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000534s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000240s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.003152s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000806s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000282s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000857s ]
