Western blotting analysis using PDLIM1 antibody (Cat#64976). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PDLIM1 antibody (Cat#64976, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using PDLIM1 antibody (Cat #64976). PDLIM1 expression in wild-type (WT) and PDLIM1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PDLIM1 antibody (Cat #64976, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat #201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat #716).
PDLIM1; PDZ And LIM Domain 1; CLP36; CLP-36; HCLIM1; Elfin; CLIM1; Carboxyl Terminal LIM Domain Protein 1; PDZ And LIM Domain Protein 1; LIM Domain Protein CLP-36; Epididymis Secretory Protein Li 112; C-Terminal LIM Domain Protein 1; PDZ And LIM Domain 1 (Elfin); HEL-S-112
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500
Note
This product is for research use only.
Data
Western blotting analysis using PDLIM1 antibody (Cat#64976). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PDLIM1 antibody (Cat#64976, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using PDLIM1 antibody (Cat #64976). PDLIM1 expression in wild-type (WT) and PDLIM1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PDLIM1 antibody (Cat #64976, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat #201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat #716).
Western blotting analysis using PDLIM1 antibody (Cat#64976). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PDLIM1 antibody (Cat#64976, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using PDLIM1 antibody (Cat #64976). PDLIM1 expression in wild-type (WT) and PDLIM1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PDLIM1 antibody (Cat #64976, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat #201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat #716).
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