Western blotting analysis using PARK7 antibody (Cat#64982). Total cell lysates (10 μg for H9c2, and 30 μg for others) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PARK7 antibody (Cat#64982, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using PARK7 antibody (Cat #64982). PARK7 expression in wild-type (WT) and PARK7 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PARK7 antibody (Cat #64982, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat #201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat #226).
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:200-1:1,000
Note
This product is for research use only.
Data
Western blotting analysis using PARK7 antibody (Cat#64982). Total cell lysates (10 μg for H9c2, and 30 μg for others) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PARK7 antibody (Cat#64982, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using PARK7 antibody (Cat #64982). PARK7 expression in wild-type (WT) and PARK7 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PARK7 antibody (Cat #64982, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat #201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat #226).
Western blotting analysis using PARK7 antibody (Cat#64982). Total cell lysates (10 μg for H9c2, and 30 μg for others) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with PARK7 antibody (Cat#64982, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using PARK7 antibody (Cat #64982). PARK7 expression in wild-type (WT) and PARK7 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with PARK7 antibody (Cat #64982, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat #201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat #226).
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