Western blotting analysis using CLIC4 antibody (Cat#64993). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CLIC4 antibody (Cat#64993, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using CLIC4 antibody (Cat #64993). CLIC4 expression in wild-type (WT) and CLIC4 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CLIC4 antibody (Cat #64993, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat #201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat #226).
Immunohistochemistry was performed on paraffin-embedded human melanoma using chloride intracellular channel 4 antibody (Cat#64993, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Applications Tested: Western blotting (WB), immunohistochemistry-paraffin (IHC-P)
Immunogen
A synthesized peptide derived from human CLIC4
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500 Immunohistochemistry-Paraffin (IHC-P): 1:100-1:200
Note
This product is for research use only.
Data
Western blotting analysis using CLIC4 antibody (Cat#64993). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CLIC4 antibody (Cat#64993, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using CLIC4 antibody (Cat #64993). CLIC4 expression in wild-type (WT) and CLIC4 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CLIC4 antibody (Cat #64993, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat #201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat #226).
Immunohistochemistry was performed on paraffin-embedded human melanoma using chloride intracellular channel 4 antibody (Cat#64993, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
Western blotting analysis using CLIC4 antibody (Cat#64993). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with CLIC4 antibody (Cat#64993, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using CLIC4 antibody (Cat #64993). CLIC4 expression in wild-type (WT) and CLIC4 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with CLIC4 antibody (Cat #64993, 1:1,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat #201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat #226).
Immunohistochemistry was performed on paraffin-embedded human melanoma using chloride intracellular channel 4 antibody (Cat#64993, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar, 25 μm.
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