Western blotting analysis using MAPK14 antibody (Cat#65006). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MAPK14 antibody (Cat#65006, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using MAPK14 antibody (Cat #65006). MAPK14 expression in wild-type (WT) and MAPK14 shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with MAPK14 antibody (Cat #65006, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat #201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat #226).
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500
Note
This product is for research use only.
Data
Western blotting analysis using MAPK14 antibody (Cat#65006). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MAPK14 antibody (Cat#65006, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using MAPK14 antibody (Cat #65006). MAPK14 expression in wild-type (WT) and MAPK14 shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with MAPK14 antibody (Cat #65006, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat #201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat #226).
Western blotting analysis using MAPK14 antibody (Cat#65006). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MAPK14 antibody (Cat#65006, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using MAPK14 antibody (Cat #65006). MAPK14 expression in wild-type (WT) and MAPK14 shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with MAPK14 antibody (Cat #65006, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat #201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat #226).
SHOW FULL COLUMNS FROM `goods` [ RunTime:0.000773s ]
SELECT * FROM `goods` WHERE `code` = '65006' LIMIT 1 [ RunTime:0.000474s ]
SHOW FULL COLUMNS FROM `goods_dict` [ RunTime:0.000426s ]
SELECT `pid`,`rank`,`title`,`desc`,`feature`,`remark`,`logo`,`id` FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000358s ]
SHOW FULL COLUMNS FROM `goods_album` [ RunTime:0.000467s ]
SELECT * FROM `goods_album` WHERE `goods_id` = 8469 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000404s ]
SHOW FULL COLUMNS FROM `goods_quotation` [ RunTime:0.000491s ]
SELECT * FROM `goods_quotation` WHERE `goods_id` = 8469 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000321s ]
SHOW FULL COLUMNS FROM `goods_parameter` [ RunTime:0.000359s ]
SELECT `title`,`short`,`id` FROM `goods_parameter` [ RunTime:0.000246s ]
SELECT * FROM `goods` WHERE `first_sort_id` = 1 AND `is_del` = 1 ORDER BY rand() LIMIT 10 [ RunTime:0.055388s ]
SELECT `goods_id`,`url`,`desc`,`sort`,`id` FROM `goods_album` WHERE `goods_id` IN (5613,4766,4316,220,7206,5032,6643,9677,103,9333) AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000823s ]
SHOW FULL COLUMNS FROM `rule_column` [ RunTime:0.000472s ]
SELECT `title`,`id` FROM `rule_column` WHERE `id` = 8 [ RunTime:0.000213s ]
SHOW FULL COLUMNS FROM `rule_site` [ RunTime:0.000416s ]
SELECT * FROM `rule_site` ORDER BY `id` DESC LIMIT 1 [ RunTime:0.000222s ]
SELECT * FROM `goods_dict` WHERE `rank` = 1 AND `is_del` = 1 ORDER BY `sort`,`id` [ RunTime:0.000279s ]
SHOW FULL COLUMNS FROM `service_sort` [ RunTime:0.000371s ]
SELECT * FROM `service_sort` WHERE `is_del` = 1 ORDER BY `sort` [ RunTime:0.000238s ]
SELECT `title`,`id` FROM `rule_column` WHERE `is_del` = 1 [ RunTime:0.000202s ]
