Western blotting analysis using SH3GL1 antibody (Cat#65029). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with SH3GL1 antibody (Cat#65029, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using SH3GL1 antibody (Cat#65029). SH3GL1 expression in wild-type (WT) and SH3GL1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with SH3GL1 antibody (Cat#65029, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HeLa cells using SH3GL1 antibody (Cat#65029, 1:1,000), Top panel: wild-type (WT); Bottom panal: SH3GL1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; SH3GL1 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Flow cytometric analysis of SH3GL1 expression in HepG2 cells using SH3GL1 antibody (Cat#65029, 1:1,000). Green, isotype control; red, SH3GL1.
Validation of SH3GL1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with SH3GL1 antibody (Cat#65029, 1:2,000) and analyzed using BD flow cytometer.
