Western blotting analysis using MAPRE2 antibody (Cat#65036). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MAPRE2 antibody (Cat#65036, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using MAPRE2 antibody (Cat#65036). MAPRE2 expression in wild-type (WT) and MAPRE2 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with MAPRE2 antibody (Cat#65036, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of MAPRE2 expression in HeLa cells using MAPRE2 antibody (Cat#65036, 1:1,000). Green, isotype control; red, MAPRE2.
MAPRE2; Microtubule Associated Protein RP/EB Family Member 2; RP1; EB2; EB1; Microtubule-Associated Protein RP/EB Family Member 2; APC-Binding Protein EB1; APC-Binding Protein EB2; End-Binding Protein 2; T-Cell Activation Protein, EB1 Family; CSCSC2
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human MAPRE2
Isotype
Mouse IgG2a
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for 1 year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:100-1:1,000
Note
This product is for research use only.
Data
Western blotting analysis using MAPRE2 antibody (Cat#65036). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MAPRE2 antibody (Cat#65036, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using MAPRE2 antibody (Cat#65036). MAPRE2 expression in wild-type (WT) and MAPRE2 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with MAPRE2 antibody (Cat#65036, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of MAPRE2 expression in HeLa cells using MAPRE2 antibody (Cat#65036, 1:1,000). Green, isotype control; red, MAPRE2.
Western blotting analysis using MAPRE2 antibody (Cat#65036). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with MAPRE2 antibody (Cat#65036, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using MAPRE2 antibody (Cat#65036). MAPRE2 expression in wild-type (WT) and MAPRE2 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with MAPRE2 antibody (Cat#65036, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of MAPRE2 expression in HeLa cells using MAPRE2 antibody (Cat#65036, 1:1,000). Green, isotype control; red, MAPRE2.
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